Contact an Account ManagerDetermination of wine amines by HPLC using automated precolumn derivatization with o-phthalaldehyde and fluorescence detection
Summary
Type: HPLC
Application #: 101310
Catalog#: 720014.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x250mm
Detection: fluorescence: excitation 230nm, emission 440nm
Conditions: eluent: 0.08M acetic acid - acetonitrile, 70:30 (v/v) for 3.5min, then gradient to 65% ACN in 6.5min, to 72% ACN in 11min, to 80% ACN in 1min, finally 3min at 80% ACN,
flow rate 1ml/min
Authors: Lehtonen, P. et al.
Source: Z. Lebensm. Unters. Forsch. 194 (1992) 434–437
Details
Legend: Chromatogram of a sample of red wine,
histamine,
tyramine,
putrescine,
b-phenethylamine,
cadaverine,
isoamylamine,
IS heptylamine (int. std.).